Protein Analysis
Tip: Reduce Western Blot Background (Mock Tip)
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DBC
Dr. Ben Carter
Center for Genomic Research
To reduce background noise on your Western Blots, try increasing the number of washes (e.g., 5 washes for 5 mins each) and adding 0.1% Tween-20 to your TBST wash buffer. A fresh blocking solution also helps! Using high-quality antibodies is key.
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4.9 / 5
Reviews15
SharedMarch 22nd, 2026