Protocol Repository
Discover, share, and improve research methods.
CRISPR-Cas9 Gene Editing in HEK293T Cells (Mock Protocol)
DER
Dr. Evelyn Reed
A detailed protocol for high-efficiency genome editing of human embryonic kidney 293T cells using the CRISPR-Cas9 system. Based on methods from Zhang Lab.
152
(28)
Standard Western Blotting for Protein Detection (Mock Protocol)
DBC
Dr. Ben Carter
A standard protocol for separating proteins by size via gel electrophoresis, transferring them to a membrane, and identifying a specific protein using antibodies. From protocols.io.
230
(45)